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CD Bioparticles is pleased to announce a suite of mRNA-LNP Vaccine Laboratory Process Development Assays.
CD Bioparticles, a leading manufacturer and supplier of numerous drug delivery products and services, is pleased to announce a suite of comprehensive mRNA-LNP Vaccine Laboratory Process Development Assays. This latest addition to CD Bioparticles’ extensive service portfolio is specifically designed for the rapid and efficient development of mRNA-LNP vaccines.
The mRNA molecule is well known to the public through COVID-19 and the mRNA vaccine made from it is characterized by rapid blocking of pathogen mutation response, simple production process and scale-up. Detection and validation methods are the basis for quality control of mRNA LNP vaccines. Therefore, R&D companies should establish detection methods for the characteristics of mRNA, liposomes and formulations, as well as related impurities and other key quality attributes of mRNA vaccines to ensure the safety and efficacy of mRNA-LNP vaccines.
CD Bioparticles now offers a comprehensive portfolio of laboratory assays designed to support every stage of mRNA-LNP vaccine development. These assays provide researchers with the tools they need to optimize their vaccine formulations and ensure their safety and efficacy, accelerating the path to clinical trials and commercialization. In addition, CD Bioparticles’ team of scientific experts is available to work with researchers to develop custom assays to meet their specific needs.
CD Bioparticles mRNA-LNP vaccine assay portfolio encompasses a variety of methodologies, including Cellular Uptake Studies,mRNA Transfection Efficiency Study, In Vivo Biodistribution, Histopathological Studies, Biochemical Indicators Testing, and Detection of Antigen-Specific T Cells. By leveraging CD Bioparticles’ expertise in analysis and characterization, researchers can gain deeper insights into the properties and performance of their vaccines, enabling them to make informed decisions during the development process.
For example, in the mRNA Transfection Efficiency Study, DC2.4 cells are selected as the model cells for the infection experiment. After infecting the cells with GFP-mRNA-LNP, the intracellular GFP signal will be observed by fluorescence microscopy. CD Bioparticles can assist customers with immunostaining studies that not only examine the efficiency of mRNA transmission, but also help to identify the specific cell types that are transfected into a specific type of organ.
CD Bioparticles also offers In Vivo Biodistribution services. To visualize the distribution of LNP in an organism, the professionals use fluorescent dyes (e.g., DiD and DiO) and luciferase for labeling. Typically, luciferase mRNA (Luc-mRNA) is used as a model mRNA to follow the distribution of the expressed protein in vivo. After intravenous injection of Luc-mRNA-LNP, heart, liver, spleen, lung, kidney and lymph node tissues are isolated. The tissues will be visualized using the IVIS Spectral Live Image System. This will facilitate visualization studies of organ distribution and duration of protein activity production.
CD Bioparticles’ new mRNA-LNP assays are available to researchers and vaccine developers worldwide. The company is committed to providing cutting-edge technologies and services that support the advancement of biotechnology and improve human health. For more information about the services, please visit https://www.cd-bioparticles.net/services/bioparticles-analysiscand-characterization/mrna-lnp-vaccine-laboratory-process-development-assay.
About CD Bioparticles
CD Bioparticles is an established drug delivery company that provides customized solutions for developing and manufacturing novel biocompatible drug delivery systems. It specializes in various formulation and drug delivery technologies, from conventional liposomes and PEGylated liposomes to polymer microspheres and nanoparticles for drug delivery. The company also provides contract research services for drug delivery formulation, formulation feasibility study, process development and scale-up, as well as analytical and non-clinical research services.
CD Bioparticles proudly announces the launch of its new Functional Agarose Particles.
With years of experience in the pharmaceutical and life science sectors, CD Bioparticles proudly announces the launch of its new Functional Agarose Particles designed for a variety of applications in the field of bioconjugation, isolation, and purification of biomacromolecules. These particles are ideal for researchers working in areas such as protein purification, antibody production, and nucleic acid isolation.
CD Bioparticles offers a range of functional agarose particles that can be used to covalently capture tagged biomolecules, such as tagged proteins, or to immobilize proteins and antibodies. They can also serve as hydrophobic chromatographic separation media, immobilized metal ion affinity chromatography resins, ion exchange chromatography resins, etc., which are suitable for conjugation of antibodies, streptavidin, enzymes and nucleic acids to activated agarose particles. They also play a critical role in the production of recombinant proteins and the purification of monoclonal antibodies. They are an important carrier tool in molecular biology and medical research, and their importance extends to biopharmaceutical and industrial engineering applications.
The new functional agarose particles are available with a variety of functional groups, including hydrophobic, ion exchange, and affinity chromatography groups. This allows researchers to choose the particles that best suit their specific needs. For example, the hydrophobic interaction chromatography (HIC) separation particles (functionalized with butyl, phenyl, octyl) can be utilized to separate proteins based on their hydrophobicity, while the immobilized metal affinity chromatography (IMAC) particles (functionalized with Al-NTA, Co2+, Co-IDA, Co-NTA, Cu-NTA, Fe-NTA, IDA, Ni2+, Ni-IDA, etc.) can be used to purify and enrich proteins such as His-tagged proteins.
In addition, agarose particles functionalized with DEAE, sulfopropyl, carboxymethyl, quaternary ammonium can be used as IEC resins with high ion exchange capacity and less nonspecific adsorption, which can be applied in the laboratory preparation of biomolecules such as proteins, nucleic acids and peptides. Activated agarose particles (functionalized with azide, alkyne, methyltetrazine, TCO, disulfide, etc.) can also be used for coupling of biomolecules.
The new functional agarose particles are available in a variety of sizes, ranging from 25 µm to 400 µm. In addition to their wide range of functionalities and sizes, these functional agarose particles offer a unique combination of properties that make them ideal for a broad range of applications, including excellent chemical stability, high yield and customizability. These particles can selectively bind to specific biomolecules, and this targeted binding capability streamlines the purification process, reducing time and effort for researchers.
CD Bioparticles’ Functional Agarose Particles are available in a variety of configurations to meet the specific needs of each research project. These particles offer superior efficiency and selectivity, enabling researchers to achieve faster and more accurate results. The company also offers custom bead development services to create particles tailored to unique applications. For more information, please visit https://www.cd-bioparticles.com/products/functional-agarose-particles-1065.html.
About CD Bioparticles
CD Bioparticles is a leading manufacturer and supplier of various nanoparticles, microparticles, and coatings for R&D as well as commercialization across different application areas, including in vitro diagnostics, biochemistry, cellular analysis, cell separation, and immunoassay. The company also offers various custom services, including chemical surface-functionalization, fluorescent modification, antibody immobilization, as well as nucleic acid and oligo conjugation to meet client specifications.
A nanometer (nm) is a unit of length that refers to one billionth of a meter. Nanotechnology is the technology that studies the properties and interactions of matter at the nanoscale (between 1 and 1000 nm) and uses these properties. In nanotechnology, nanomaterials are its main research object and foundation. CD Bioparticles offers a variety of synthetic nanoparticles and magnetic particles to provide researchers with a wealth of high-quality nanomaterials for research use.
In fact, nanotechnology is not mysterious, nor is it a human patent. As early as the birth of the universe, nanomaterials and nanotechnology already existed. During the long evolution on the earth, many creatures in nature are masters of many nanotechnologies. They survive tenaciously in nature through their exquisite nano-skills so that they can make a living, or defend themselves against enemies, which not only impresses people but also brings countless inspiration and inspiration to modern nanotechnology workers.
Lotus flower
When it comes to lotus, people naturally think of the dew rolling on the lotus leaf, the so-called lotus effect. So, what causes this lotus effect? What benefits does the lotus effect bring to the lotus itself? Modern electron microscope technology can help us give the right answer. Through an electron microscope, it can be observed that the surface of the lotus leaf is covered with countless 10-micrometer mastoids, and the surface of each mastoid is covered with finer hairs with a diameter of only several hundred nanometers. This is the result of the long-term evolution of organisms in nature. It is this special nanostructure that makes the surface of lotus leaves free of water droplets, namely the ultrahydrophobicity. The lotus effect keeps the leaves clean. When there are water droplets on the lotus leaf, the wind blows the water droplets to roll on the leaf surface. Dirt particles are picked up by water droplets and slide down from above at a high speed so that the lotus leaf can perform photosynthesis better.
Serpent sea star
The serpent sea star is a dish-shaped shellfish. It has five tentacles and no eyes. Nevertheless, it can accurately sense potential natural enemies in the distance and retract the tentacles into the shell in time. This sensitive feeling of serpent sea star has long puzzled biologists. Recently, this question has finally found the answer on its carapace: the serpent sea star body is actually covered with “eyes”, that is, tens of thousands of perfect miniature lenses. In this way, the entire furry body constitutes the starfish’s eyes.
Studies also show that the number of such lenses on a serpent sea star is about 50,000 to 100,000, and they are composed of calcium carbonate nanocrystals; this perfect light-sensitive micro-lens system is the result of nano-crystallization on the surface of the starfish’s body growth. In order to prevent unnecessary color fringing, a proper amount of magnesium is also absorbed in the lens during crystallization, which can not only help starfish filter the light more effectively but also correct the “spherical aberration” of the lens. This improves the efficiency of finding natural enemies.
Spider
Spider webs often appear in the corners of rooms that have not been cleaned for a long time. For ordinary people, cobwebs are not a big deal. With a slight flick, the spider webs are swept away. But spider silk itself is indeed a miracle of nature. Spider silk in nature is about 100 nanometers in diameter, which is a truly pure natural nanofiber. If you use spider silk to make a rope as thick as an ordinary wire rope, it can lift thousands of tons of objects, and its strength is comparable to steel cables.
In addition to catching flying insects, almost all spiders also use spider silk as a direction, safety rope, and gliding rope. Spiders usually have several glands on their abdomen, called spinnerets. Various glands produce different types of spider silk. There is a spinneret at the top of the gland, which has thousands of small holes, and the sprayed liquid will condense into cohesive, high-tension spider silk upon encountering air. Spider silk consists of spidroin proteins. Usually, a thousand strands of spider silk are still thinner than 1/10 of a human hair.
Bee Studies have shown that many organisms, including bees and turtles, have nano-sized magnetic particles in their bodies. These magnetic nanoparticles are of great significance for the localization and movement behavior of living things. For example, there are magnetic nanoparticles in the abdomen of bees. Such magnetic particles have the function of a compass. Bees use this “compass” to determine their surrounding environment and use images stored in magnetic nanoparticles to determine the direction. When the bees come back from collecting honey, they are actually comparing the original stored image with the image they saw until the two images are consistent, thereby judging their own hive. Using this magnetic nanoparticle for navigation, bees can complete miles of the journey.
Resource from https://www.cd-bioparticles.com/blog/nanoparticles/nanotechnology-in-nature/